Fill in the sequence in the flow of genetic information, regularly called the central dogma. Placed the name of the process over each arrow.

You are watching: What determines if a ribosome becomes bound to the er

_________ → _________ → _________


Practice making use of the dictionary of the hereditary cocde in your textbook. Determine the amino acid sequence for a polypeptide coded for by the complying with mRNA tranmanuscript (created 5" → 3"):

AUGCCUGACUUUAAGUAG


a. Initiation: Transcription factors bind to promoter and also facilitate the binding of RNA polymerase II, developing a transcription initation complex; RNA polymerase II sepaprices DNA strands at initation site.

b. Elongation: RNA polymerase II moves along DNA strand also, connecting RNA nucleotides that have actually paired to the DNA template to the 3" finish of the flourishing RNA strand.

c. Termination: After polymerase transcribes past a polyadenylation signal sequence, the pre-mRNA is cut and released.


A 5" cap consisting of a modified guanine nucleotide is added to the 5" UTR. A poly-A tail consisting of up to 250 adenine nucleotides is attached to the 3" UTR. Spliceosomes have cut out the introns and spliced the exons together.


Using some of the codons and the amino acids you established in Interactive Question 17.2, fill in the attached table.

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In the adhering to diagram of polypeptide synthesis, name the stage, briefly describe what happens in the stage, and recognize the component (a-f).

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Codon recognition: An elongation variable (not shown) helps an aminoacyl tRNA into the A site wright here its anticodon base-pairs to the mRNA codon; 2 GTP increase accuracy and performance.

a. amino finish of thriving polypeptide

b. aminoacyl tRNA

c. large subunit

d. A site

e. small subunit

f. 5" finish of mRNA


In the adhering to diagram of polypeptide synthesis, name the stage, briefly describe what happens in the stage, and also recognize the component (g).

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Peptide bond formation: Ribosome catalyzes peptide bond formation between new amino acid and polypeptide held in the P website.

g. peptide bond formation


In the complying with diagram of polypeptide synthesis, name the stage, briefly define what happens in the stage, and also identify the component (h).

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Translocation: The empty tRNA in the P website is moved to the E website and also released; the tRNA now holding the polypeptide is relocated from the A to the P website, taking the mRNA with it; one GTP is forced.

h. E site


In the complying with diagram of polypeptide synthesis, name the phase, briefly describe what happens in the stage, and identify the component (i-l).


Termination: Release aspect binds to soptimal codon in the A site. Free polypeptide is released from the P website. Ribosomal subdevices and various other assembly components sepaprice.

i. release factor

j. sheight codon

k. P site of

l. totally free polypeptide


Define the following, and also define what form of allude mutation could reason each of these mutations.

a. silent mutation

b. misfeeling mutation

c. nonfeeling mutation

d. frametransition mutation


a. Silent: a base-pair substitution producing a codon that still codes for the same amino acid.

b. Missense: a base-pair substitution or frametransition mutation that outcomes in a codon for a different amino acid.

c. Nonsense: a base-pair substitution or framechange mutation that creates a sheight codon and prematurely terminates translation.

d. Frameshift: an insertion or deletion or one, 2, or even more than three nucleotides that disrupts the reading framework and also creates considerable missense and also nonsense mutations.


12

In Beadle and Tatum"s research of Neurospora, they were able to identify 3 classes of mutants that necessary arginine included to the minimal media in order to grow. The manufacturing of arginine consists of the adhering to steps: precursor → ornithine → citrulline → arginine. What nutrient(s) had actually to be supplied for the mutants with a defective enzyme for the ornithine → citrulline step to grow?

a. the precursor

b. ornithine

c. citrulline

d. either ornithine or citrulline

e. the precursor, ornithine, and also citrulline


c. citrulline


13

Transcription entails the carry of information from

a. DNA to RNA.

b. RNA to DNA.

c. mRNA to an amino acid sequence.

d. DNA to an amino acid sequence.

e. the nucleus to the cytoplasm.


a. DNA to RNA.


14

If the 5" → 3" nucleotide sequence on the complementary (noncoding) DNA strand is CAT, what is the matching codon on mRNA?

a. UAC

b. CAU

c. GUA

d. GTA

e. CAT


b. CAU


15

RNA polymerase

a. is the protein responsible for the manufacturing of ribonucleotides.

b. is the enzyme that creates hydrogen bonds in between nucleotides on the DNA layout strand and the complementary RNA nucleotides.

c. is the enzyme that transcribes exons however does not transcribe introns.

d. is a ribozyme created of snRNPs.

e. moves along the layout strand also of DNA, elongating an RNA molecule in a 5" →3" direction.


e. moves along the theme strand also of DNA, elongating an RNA molecule in a 5" →3" direction.


16

How is template strand for a certain gene determined?

a. It is the DNA strand also that runs from the 5" → 3" direction.

b. It is the DNA strand that runs from the 3" → 5" direction.

c. It is established within the promoter.

d. It doesn"t matter which strand also is the layout because they are complementary and wil produce the same mRNA.

e. It is signaled by a polyadenylation signal sequence.


c. It is establimelted within the promoter.


17

Which enzyme sythesizes tRNA?

a. RNA replicase

b. RNA polymerase

c. aminoacyl-tRNA synthetase

d. ribosomal enzymes

e. ribozymes


b. RNA polymerase


18

Which of the complying with is true of RNA processing?

a. Exons are excised before the mRNA is translated.

b. The RNA transcipt that leaves the nucleus might be a lot longer than the original transcript.

c. Assemblies of protein and snRNP"s, referred to as spliceosomes, might catalyze splicing.

d. Large quantities of rRNA are assembled into ribosomes.

 e. Signal peptides are included to the 5" end of the transcript.


c. Assemblies of protein and snRNP"s, dubbed spliceosomes, might catalyze splicing.


19

Which of the adhering to is not associated in the development of a eukaryotic transciption initiation complex?

a. TATA box

b. transcriptionn factors

c. snRNA

d. RDA polymerase II

e. promoter


c. snRNA


20

A prokaryotic gene 600 nucleotides lengthy can code for a polypeptide chain of just how many kind of amino acids (at most)?

a. 100

b. 200

c. 300

d. 600

e. 1,800


b. 200


21

All of the following are transcribed from DNA except

a. exons.

b. introns.

c. tRNA.

d. rRNA.

e. promoter


e. promoter


22

What can introns have to perform through the development of new proteins?

a. The excised introns are transcribed and also analyzed as new proteins by themselves.

b. Introns are more most likely to accumulate mutations than exons, and also these mutations then lead to the production of novel proteins.

c. Introns that are self-excising might additionally attribute as hydrolytic enzymes for various other procedures.

d. Introns administer more location where crossing over might happen (without interfering through the coding sequences) and also thus increase the probability of exon shuffling between alleles.

e. Introns regularly correspond to domains in proteins that fold individually and also have specific functions. Switching domain names between nonallelic genes can develop novel proteins.


d. Introns carry out even more location wbelow crossing over may take place (without interfering through the coding sequences) and also hence increase the probcapacity of exon shuffling between alleles.


23

A ribozyme is

a. an exception to the one gene–one RNA molecule axiom.

b. an enzyme that adds the 5" cap and poly-A tail to an mRNA.

c. an example of resetup of protein domain names caused by RNA splicing.

d. an RNA molecule that attributes as an enzyme.

e. an enzyme that produces both small and also big ribosomal subdevices.


d. an RNA molecule that functions as an enzyme.


24

All of the adhering to would be discovered in a prokaryotic cell except

a. mRNA.

b. rRNA.

c. simultaneous transcription and translation.

d. snRNA.

e. RNA polymerase.


d. snRNA.


25

Which of the following is transcribed and then interpreted to create a protein product?

a. gene for tRNA

b. intron

c. gene for a transcription factor

d. 5" and 3" UTRs

e. gene for rRNA


c. gene for a transcription factor


26

Transfer RNA

a. forms hydrogen bonds in between its codon and also the anticodon of an mRNA in the A site of a robosome.

b. binds to its certain amino acids in the active website of a aminoacyl-tRNA synthetase.

c. has catalytic actvity and is thus a ribozyme.

d. is translated from mRNA.

e. is developed inthe nucleolus.


b. binds to its certain amino acids in the active website of a aminoacyl-tRNA synthetase.


27

Place the following events in the synthesis of a polypeptide in the appropriate order.

1. a peptide bond creates.

2. An aminoacyl tRNA mathces its anticodon the the codon in the A side. 

3. At tRNA translocates from the A to the P site, and an unattached tRNA leaves the ribosome from the E side.

4. The large subunit attaches to the small subunit, with the initiator tRNA and the P site.

5. A little subunit binds to an mRNA and also an initiator tRNA.

a. 4-5-3-2-1

b. 4-5-2-1-3

c. 5-4-3-2-1

d. 5-4-1-2-3

e. 5-4-2-1-3


e. 5-4-2-1-3


28

Transplace in the procedure of translation involves

a. the hydrolysis of a GTP molecule.

b. the movement of the tRNA in the A website to the P website.

c. the movement of the mRNA strand one triplet length.

d. the release of the unattached tRNA from the E website.

e. every one of the above.


e. all of the above.


29

Which of the adhering to kind of molecule catalyzes the development of a peptide bond?

a. RNA polymerase

b. rRNA

c. mRNA

d. aminoacyl-tRNA synthetase

e. proteinase


b. rRNA


30

Which of the complying with is not true of an anticodon?

a. It is composed of three nucleotides.

b. It lines up in the 5" → 3" direction along the 5" → 3" mRNA strand.

c. It exoften tends from one loop of a tRNA molecule.

d. It might pair with even more than one codon.

e. Its base uracil base-pairs via adenine.

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b. It lines up in the 5" → 3" direction alengthy the 5" → 3" mRNA strand.


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