OVERVIEWA 10M sodium hydroxide (NaOH) stock solution is provided for plenty of applications consisting of adjusting the pH of assorted solutions.The Normality the NaOH equipment is equal to the molarity the the solution. This method that the normality the a 10M equipment of NaOH is equal to 10N.The molecular load of NaOH is 40. That method you need to dissolve 40 g of NaOH in water to achieve a 1 liter of 1M (or 1N) NaOH solution. To prepare a 10M NaOH solution, you need to dissolve 10 times much more NaOH i.e., 400 g of NaOH for 1 l solution.Here we will prepare 100 ml that 10M NaOH solution. Therefore, we should take 40 g that NaOH.Dissolution of NaOH is a hyperthermic reaction (generates heat), therefore, NaOH is added in water in tiny quantities rather of including all in ~ a time to border the warm generation as too much heat can damages the container (glass beaker).
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Reagents and solutionsSodium hydroxide (NaOH) palletDeionized / twin distilled water
Equipment and disposablesMeasuring cylinder/volumetric flaskErlenmeyer flask / BeakerGlass rod/Magnetic stirrerIce bucket with ice (optional)Weighing Balance
Composition10 M NaOH solution
ObjectivePreparation that 100 ml that 10 M NaOH equipment in water
Use personal protective tools (lab coat, gloves, goggles etc) for her safety and also follow the accuse of your institute.
Step 1: to prepare 100 ml of 10 M NaOH solution, sweet out 40 g the NaOH (molecular weight: 40).
Precaution:Don’t reveal solid NaOH come air because that a lengthy time if weighing. NaOH is hygroscopic in nature and absorbs water native the setting quickly.
Step 2: Dissolve NaOH pellets in 70 ml water through adding little quantities that NaOH in the water.◊ take 70 ml deionized/double distilled water in a beaker.◊ add a few pellets (4 – 5 pellets every time) the NaOH in water and wait till they dissolve. Repeat this until all 40 g NaOH pellets are dissolved in water.
Precautions: 1. The dissolution of NaOH is a highly exothermic reaction, i beg your pardon can also break the glass beakers. That is recommended to store the maker on ice cream while dissolve NaOH.2. Never include 70 ml water to 40 g NaOH the pellet.
Step 3: readjust the solution volume come 100 ml through deionized/double distilled water.◊ Transfer solution to a measuring cylinder/volumetric flask (capacity: 100 ml) and readjust the volume to 100 ml with deionized/double distilled water.
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Storage:The solution have the right to be stored at room temperature for numerous months.
Note:There is no have to sterilize this solution by autoclaving. No virus will grow in concentrated NaOH solution.
Applications● NaOH is a solid base and commonly provided to change the pH of countless solutions.● Plasmid isolation by alkaline lysis method● preparation of EDTA solution
|Follow the table to prepare NaOH solution of certain concentration and also volume|
|Conc. / Volume||50 ml||100 ml||250 ml||500 ml|
|1 M||2.00 g||4.00 g||10.00 g||20.00 g|
|2 M||4.00 g||8.00 g||20.00 g||40.00 g|
|5 M||10.00 g||20.00 g||50.00 g||100.00 g|
|10 M||20.00 g||40.00 g||100.00 g||200.00 g|
Use calculator to calculation the quantities of NaOH for the preparation of NaOH systems of details concentration and also volume